iTOL annotation editor

Access your iTOL trees directly from Microsoft Excel, and annotate them using familiar spreadsheet interface.


Simply create new datasets or edit exiting ones

Load existing annotations directly from iTOL, or start from scratch. Preload tree identifiers from iTOL, and never worry about finding the correct ID to use.


Full error reporting directly in Excel

Any errors in your data will be highlighted directly in Excel, with helpful information popups. Use the error navigator to quickly go from one error cell to the next.


Helpful tooltips and value selectors

Tooltips describe each field, making it easy to fill the correct values. Where applicable, values can be directly selected from a list of valid entries.


Integrated color picker

Type the colors directly or use the integrated color picker. Cell backgrounds change to reflect the selected color.


iTOL annotation editor

iTOL annotation editor is a Microsoft Excel spreadsheet providing direct link to your iTOL account. Simply access your trees directly from Excel, create new datasets, edit existing ones, define color ranges or update tree labels.

Edit datasets

Create or edit datasets directly in Excel, with full link to iTOL. Automatically load your tree node IDs, and save data directly to iTOL.

Define color ranges

Use the integrated color picker to define colors in datasets or colored ranges. Cell backgrounds are automatically set to reflect the selected color.

Error reporting

Get feedback on errors in your data directly in Excel. Invalid cells are highlighted and show helpful hints on the detected errors. Use the Error navigator to quickly navigate among all invalid cells.


Full power of Microsoft Excel tied directly with iTOL's robust tree annotation features.

Full access to iTOL's most popular annotation features.

Create datasets, load tree IDs directly from iTOL and start working on your data. Save from Excel automatically into iTOL, without need for separate files or templates. Load your tree in iTOL's web page to visualize the data.


Helpful info popups and automatic data validation.

Descriptions of various fields give helpful hints directly in the Excel interface. Values for pre-defined field types can be selected directly through pop-down value lists.

Simple error correction

Error navigator simplifies the identification and correction of any problems in your data. All error cells are automatically highlighted and show the detected error when selected.


Video tutorial

An in-depth video tutorial, together with the description of all features and supported datasets is available on the help page.


iTOL annotation editor works in trial mode by default, and will only save the first five rows of your data. For full unlimited access, subscribe using one of the options below. For institutional access, please have your librarian or IT administrator contact us.


limited access


  • All features available
  • No time limit
  • Only first 5 rows of data are saved in iTOL
  • Use it for testing on replaceable trees and data
Download below

30 days

Unlimited access


  • Full unlimited access
  • Email support
  • Data saved in iTOL remains permanently accessible
  • Does NOT extend automatically
Subscribe in iTOL

1 year

Unlimited access


  • Full unlimited access
  • Email support
  • Data saved in iTOL remains permanently accessible
  • Does NOT extend automatically
Subscribe in iTOL


Various models available


  • Access for the whole organization
  • Email support
  • Flexible models
  • Network range access possible
Email for details


Download the latest version of the iTOL annotation editor:

1.1iTOL editor v1_1.xlsmFull version of the editor, tested in Microsoft Office 2019.
1.1iTOL editor for Excel 2011 v1_1.xlsmSome non-essential functionality was removed to make it work in older versions of Excel. Use this one only if the full version doesn't work.

Version history

1.1Added support for the Text labels dataset. Fixed an issue with single quotes in node IDs.
1.0initial release